Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: The characterization of two kinds of iron nanoparticles and their effects on KG1a and HL60: ( A , B ) hydrated particle size and zeta potential of Prussian blue nanoparticles (PBNPs) ( A ) and Fe 3 O 4 nanoparticles (FeNPs) ( B ), ( C ) cell viability of the PBNP- and FeNP-treated HL60 and KG1a cells, ( D ) cellular iron contents of the PBNP- and FeNP-treated HL60 and KG1a cells, and ( E ) QPCR detection of CD34 and CD38 expression in two cells. HF, FeNP-treated HL60; HP, PBNP-treated HL60; KF, FeNP-treated KG1a; KP, PBNP-treated KG1a. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001. ( F ) ROS levels of the PBNP- and FeNP-treated HL60 and KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Zeta Potential Analyzer, Expressing

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparisons of differentially expressed genes (DEGs) in two Leukemia cells treated with two kinds of iron nanoparticles: ( A ) number of upregulated and downregulated DEGs (fold change > 1.0) in the PBNP- and FeNP-treated HL60 and KG1a cells and their relationship, ( B ) common DEGs (fold change > 1.5) in a cell treated by two kinds of nanoparticles, ( C ) common DEGs (fold change > 1.5) in two cells treated by a nanoparticle, ( D ) common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles, and ( E ) schematic of cellular functions of common DEGs (fold change > 1.0) in two cells treated by two kinds of nanoparticles. The detailed information of all DEGs is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Top 20 GO terms of the KG1a cells treated with two kinds of iron nanoparticles: ( A , B ) heatmap ( A ) and enrichment network ( B ) colored by the same cluster of GO terms in the FeNP-treated KG1a cells, and ( C , D ) heatmap ( C ) and enrichment network ( D ) colored by the same cluster of GO terms in the PBNP-treated KG1a cells. The colored labels followed the order of top 20 GO terms. ( E ) Venn analysis of top 20 GO terms in the KG1a cells treated with PBNPs and FeNPs. The detailed information of all GO terms is shown in .

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of lipid metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Membrane, Activity Assay

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: GO terms of metal ion metabolisms in KG1a and HL60 exposed to FeNPs or PBNPs (Top 20, p < 0.01).

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Activity Assay, Clinical Proteomics, Membrane

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Comparative Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs ( p < 0.05): ( A ) comparative top 10 enriched pathways of HL60 and KG1a treated with FeNPs and ( B ) comparative top 10 enriched pathways of HL60 and KG1a treated with PBNPs. The pathways in black and blue were HL60 and KG1a cells, respectively. ( C ) Four-way Venn analysis of all KEGG pathways in four groups. The detailed information of all KEGG pathways is shown in . HF, FeNP-treated HL60 cells; HP, PBNP-treated HL60 cells; KF, FeNP-treated KG1a cells; and KP, PBNP-treated KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Nanomaterials

Article Title: Effects of Two Kinds of Iron Nanoparticles as Reactive Oxygen Species Inducer and Scavenger on the Transcriptomic Profiles of Two Human Leukemia Cells with Different Stemness

doi: 10.3390/nano10101951

Figure Lengend Snippet: Validation of RNA-Seq DEGs using RT-qPCR: ( A , B ) the expression levels of 10 selected DEGs in the iron nanoparticle-treated HL60 ( A ) and KG1a ( B ) cells. All values are mean ± SD with n = 3. ns, no significance; *, p < 0.05; **, p < 0.01, ***, p < 0.001, ****, and p < 0.0001. ( C , D ) The comparison of fold change detected by RT-qPCR and RNA-Seq in the iron nanoparticle-treated HL60 and ( C , D ) KG1a cells.

Article Snippet: The human acute myelogenous leukemia (AML) cell KG1a and the human acute promyelocytic leukemia (APL) cell HL60 were obtained by the China Center for Type Culture Collection (Shanghai, China).

Techniques: Biomarker Discovery, RNA Sequencing, Quantitative RT-PCR, Expressing, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Mesenchymal stromal cells (MSCs) decreased leukemic cell proliferation. Experiments were performed with KG1a leukemic cells cultured alone (blue), with MSC-CM (green), or in coculture with MSCs (red). ( A ) Experimental design of mono- and coculture of MSCs and leukemic KG1a cells. Leukemic cells were cultured in medium alone, with MSC-CM or over MSCs; MSCs were cultured alone or with KG1a leukemic cells. ( B ) Leukemic cell growth was evaluated after 72 h of mono-, MSC-CM, or coculture (nonadherent cells represent cells that do not adhere to MSCs after 72 h of coculture, n = 15). ( C ) Cell cycle was analyzed by a flow cytometry multilabeling protocol using anti-Ki67-AF488, anti-phosphoS10-H3-AF488 and 7AAD ( n = 5). Variations in MSC-CM and cocultured vs. monocultured KG1a in cell cycle phases after 72h are shown on the left and a representative experiment is presented on the right. * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Cell Culture, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Contact with MSCs increases SP proportion in leukemic cells. SP was assessed by Hoechst efflux measurement in flow cytometry. Cytograms in ( A ) illustrate a representative acquisition of Hoechst staining of KG1a leukemic cells after a 72 h culture alone (left panel), in MSC-CM (middle panel), or with MSC-contact (right panel). Quantitative results are shown as SP percentages ( B ) absolute numbers ( C ) in the three culture conditions ( n = 4). * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Flow Cytometry, Staining

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: MSCs decrease the energy metabolism of leukemic cells. Energy metabolism was assessed through the evaluation of mitochondrial respiration (OCR) and glycolysis (ECAR) and with Seahorse XFe96 ( n = 7). ( A ) Analysis of energy metabolism in KG1a cells. ( B ) Analysis of energy metabolism in MSCs. * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Interaction between leukemic cells and MSCs induces opposite oxidative metabolism and Nrf2 pathway modifications in both cell types. ( A ) Intracellular ROS level was analyzed by flow cytometry after CM-H 2 DCFDA staining in leukemic cells cultured alone (blue), with MSC-CM (green), or with MSCs (red) as well as in MSCs cultured alone (violet) or cocultured with KG1a cells (dark violet) for 72h ( n = 5–9). ( B ) The expression and activation of p38MAPK were analyzed by Western blot in KG1a cultured alone, with MSC-CM or cocultured as well as in MSCs alone or cocultured with leukemic cells ( n = 3); alpha-tubulin was used as loading control. ( C ) Nrf2 subcellular localization was analyzed by Western blot in the cytoplasmic and nuclear fractions of leukemic cells (left) or MSCs (right). RAF and TOPO-1 were used as loading controls and purity indicators of the cytoplasmic and nuclear fractions, respectively. ( D ) Nrf2 target genes expression was evaluated by transcriptomic analysis and is presented as relative expression vs. KG1a cells or MSCs alone ( n = 3). * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Flow Cytometry, Staining, Cell Culture, Expressing, Activation Assay, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Involvement of GPx-3 in the Reciprocal Control of Redox Metabolism in the Leukemic Niche

doi: 10.3390/ijms21228584

Figure Lengend Snippet: Leukemic cells and MSCs reciprocally modify their gene expression profile of antioxidant enzymes. ( A ) Gene expression of the major antioxidant enzymes was analyzed by real-time PCR in KG1a cells and is presented, from the highest to the lowest expressed genes in KG1a cells, as the percentage of increased or decreased relative expression (RQ = 2 −ΔΔ C t ) in KG1a cells cultured in MSC-CM (green dots) or cocultured with MSCs (red dots) vs. KG1a cells alone (blue line) (left). Increased GPX3 expression was studied at the protein level by Western blot analysis (right), alpha-tubulin being used as loading control. ( B ) Gene expression of major antioxidant enzymes was analyzed by real-time PCR in MSCs and is presented, from the highest to the lowest expressed gene in MSCs alone, as the percentage of increased or decreased relative expression (RQ = 2 −ΔΔ C t ) in cocultured MSCs (violet dots) vs. MSCs alone (violet line) (left). Decreased GPX3 expression was confirmed at the protein level by Western blot analysis (right), alpha-tubulin being used as loading control. * p < 0.05.

Article Snippet: The human acute myeloid leukemia cell-line KG1a (FAB M0/M1, CD34 + ) was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, UK) and cultured in Minimum Essential Medium Alpha (αMEM, Gibco BRL, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM L-glutamine (Gibco BRL), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco BRL).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Cell Culture, Western Blot, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Synthesis and biological evaluation of dithiocarbamate esters of parthenolide as potential anti-acute myelogenous leukaemia agents

doi: 10.1080/14756366.2018.1490734

Figure Lengend Snippet: Inhibitory effects of dithiocarbamate esters of parthenolide against KG1a and HL-60 cells.

Article Snippet: KG1a cells were treated with 2 μM 7l for 24 h. Gene chip assay was performed by Genergy Biotechnology Company (Shanghai, China).

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Synthesis and biological evaluation of dithiocarbamate esters of parthenolide as potential anti-acute myelogenous leukaemia agents

doi: 10.1080/14756366.2018.1490734

Figure Lengend Snippet: Compound 7l induced the apoptosis of diverse cultured leukaemia cells. (a) The representative picture of apoptosis induced by 7l in KG1a cells. (b) Apoptosis of THP-1, HL-60/ADR, K562, KG1a cells after being exposed to different concentrations of 7l for 48 h. The percentages of apoptosis were determined by flow cytometry using Annexin V/PI. (c) The representative picture of apoptosis induced by 7l and PTL in KG1a cells at 1 µM. (d) Apoptosis of KG1a cells after being exposed to 0.2, 0.5, 1 µM of 7l or PTL for 48 h. These experiments were performed for three times. Analysis results represented mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: KG1a cells were treated with 2 μM 7l for 24 h. Gene chip assay was performed by Genergy Biotechnology Company (Shanghai, China).

Techniques: Cell Culture, Flow Cytometry

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Synthesis and biological evaluation of dithiocarbamate esters of parthenolide as potential anti-acute myelogenous leukaemia agents

doi: 10.1080/14756366.2018.1490734

Figure Lengend Snippet: Preliminary mechanism study of 7l . (a) Heat map analysis of microarray data between control group and 7l -treated group in KG1a cells. (b) The KEGG enrichment analysis of microarray data between control group and 7l -treated group in KG1a cells. (c) Heat map analysis of microarray data of MAPK pathway after treatment of 7l at a concentration of 2 µM. (d) Western blot analysis of MAPK pathway related proteins and apoptosis mediated proteins after exposing to different concentrations of compound 7l for 24 h in KG1a cells.

Article Snippet: KG1a cells were treated with 2 μM 7l for 24 h. Gene chip assay was performed by Genergy Biotechnology Company (Shanghai, China).

Techniques: Microarray, Control, Concentration Assay, Western Blot